微波固定系统 (*新型号)
（实验动物脑内酵素瞬间灭活性系统）
Muromachi Microwave Fixation system
原理与特色：
1. 用微波加热的方式使得神经组织内的酶（ENZYME）去活性，以避免死后变化
（post-mortem changes）持续进展。
2. 此法比用冷冻固定法要来得好：冷冻法需要10-90秒做深入冷冻去活性，这段
期间，深部组织的死后变化（post-mortem changes）已经在进行，因脑表面
的热传导转差。微波法可在更短的时间内完全去活性。
3. 可以测：
* Acetylcholine乙酰胆碱
* Choline 胆碱
* Cyclic AMP 环腺苷酸
* Cyclic GMP 环林鸟苷
* GABA 伽马氨基丁酸
* DOPA 多巴（二羟基苯丙氨酸）
* 5-HTP 5-羟色胺酸
* Serotonin 血清素
* Endorphin 内啡肽
* Prostaglandin 前列腺素
* Catecholamines and their metabolites 儿茶酚胺及其代谢物
* Phosphorylated Proteins 磷酸化蛋白质
微波固化可以达到下列标准：
1. 可将温度升至75-90摄氏度，使脑部快速固定。
2. 微波能量可有效集中在动物的头部。
3. 各种动物间都可以达到相同的结果。
4. 对操作人员具备严格的保护，此设备相当简单**
5. CE认证，国际规定家用微波设备微波泄漏不得超越5毫瓦/立方厘米，
我们的设备**标准远远低于此，不超过1毫瓦/立方厘米。
新增特性：
1. 所有控制均位于前面板触摸屏幕上
2. 数字化使得电源级别设置更容易，重复性更强
3. 客户处电压不稳波动性得到很好的控制
4. 采用风冷装置，无需水冷，升降温时间控制速度快
5. 整体重量变轻，仅为103公斤
1. 波导管 2. 应用管头 3. 应用管门闭
4. 触摸屏 5. 主开关（断路器） 6. 空气入口
7. 脚轮 8. 应用管帽 9. 应用管门开关
10. 应用管门 11. 应用管头室 12. 空气出口
13. 电源线接口
规格
振荡器
Panasonic Magnetron 2M265-M11
微波输出
5kW max. （2-5 千瓦变频）
震荡频率
2.450±30MHz
冷却要求
空气冷却
水流量
每分钟两升
暴露时间
0.01-9.99秒 (0.01秒步进)
暂停时间
3 分钟
能量功耗
8 千瓦
频率
50 或者 60 Hz
电源要求
190-240 VAC 30A 3相 或者
380-440 VAC 20A 3相
外形尺寸
750 W x 550 D x 1,275 H 厘米
重量
大约 103公斤
|
APPLICATOR HEAD |
适用的动物管 |
| TAW-174P | WJM-24 for Mouse 15-20g WJM-28 for Mouse 20-40g |
| TAW-424SP | WJR-S for Rat 150-250g |
| TAW-424MP | WJR-M for Rat 250-400g WJR-L for Rat 400-500g |
水套式动物管（左侧的是大鼠固定器，右侧的是老鼠固定器）
|
标准系统包括： | |
| Applicator Head ( Any one from the list ) | 1 |
| Animal Holder ( Any one from the list ) | 1 |
| Dust Cover | 1 |
发表文献：
Unambiguous assignment of the H3S and H3R deuterations of cerebral (2-13C) glutamate by 13C NMR at 18.8 tesla
TB Rodrigues, IR Violante, S … - Magnetic Resonance in …, 2010 - interscience.wiley.com
... At the end of the infusions, cerebral metabolism was arrested using a 5- kW microwave
fixation system, the brain being rapidly removed from the skull and immediately frozen in liquid N2. ...
Brain glutamine synthesis requires neuronal-born aspartate as amino donor for glial glutamate formation
... regulated circulating water bath. Fifteen minutes after 13 C-label administration, the
metabolism was arrested using a high-power (5 kW) focused microwave fixation system
The brain was ...
Delayed Increase of Brain Noradrenaline After Acute Footshock Stress in Rats
... 4.5–5 kW, 1.4 s) using a small animal microwave applicator...
Immediately after microwave radiation, the brains were harvested and put onto an ice-chilled
Petri dish ... Moroji T, Takahashi T, Ogura K, Toishi T, Arai S (1977) Rapid fixation of rat ...
Altered Affect, Monoamine Transmitters and Bioenergetic Homeostasis of Alpha-synuclein-transgenic Mice, in the Presence and Absence of Endogenous Alpha- EM Cumyn - 2010 - tspace.library.utoronto.ca
... 36 3.7 Open field test..... 37 3.8 Microwave fixation and dissection of brain tissue.....
37 3.9 Fresh dissection and flash-freezing of brain tissue..... 40 ...
Brainstem Concentrations of Cholesterol are not Influenced by Genetic Ablation of the Low-Density Lipoprotein Receptor
AY Taha, CT Chen, Z Liu, JH Kim, HTJ Mount, … - Neurochemical …, 2009 - Springer
... At 7 weeks of age, the mice were euthanized with a high-energy head-focused microwave
fixation system (6 kW, 0.88–0.99 s, Muromachi brain fixation system in order to stop brain lipid metabolism [14, 15]. ...
Detection of reduced GABA synthesis following inhibition of GABA transaminase using in vivo magnetic resonance signal of [13C] GABA C1
J Yang, C Johnson, J Shen - Journal of neuroscience methods, 2009 - Elsevier
... After in vivo MRS data acquisition of gabaculine-treated rats infused with [2,5- 13 C 2 ]glucose
and unlabeled glucose, the rats were euthanized using a microwave fixation device (Model
TNW-6402C, 5 kW, 1.5 s). Subsequently, the rat ...
Brainstem Concentrations of Cholesterol are not Influenced by Genetic Ablation of the Low-Density Lipoprotein Receptor
Detection of reduced GABA synthesis following inhibition of GABA transaminase using in vivo magnetic resonance signal of [13C] GABA C1
自然基因科技有限公司**代理
电话：010-51650520
邮件：info@naturegene.com